Part 1 Basic Information
Name: Li Wang
Address: School of Pharmacy, Jiangsu University, No. 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China
Email: liwang@ujs.edu.cn
Part 2 Personal Profile
Dr. Li Wang is an Associate Professor and Master's Supervisor at the School of Pharmacy, Jiangsu University. Her research focuses on sample pretreatment technologies and CRISPR-and Ago-based detection methods. She has led 2 national and provincial research projects and published over 50 papers in peer-reviewed journals, including Analytical Chemistry, Chemical Engineering Journal, and Biosensors and Bioelectronics. She serves as a reviewer for journals such as Analytical Chemistry, Biosensors and Bioelectronics, and Talanta.
Part 3 Education
•2005.09—2008.07, Shaanxi Normal University, Analytical Chemistry, Ph.D.
•2002.09—2005.07, Shaanxi Normal University, Analytical Chemistry, M.S.
•1998.09—2002.07, Yanbei Normal University, Chemistry Education, B.S.
Part 4 Work Experience
•2008.07–2015.11, School of Pharmacy, Jiangsu University, Lecturer
•2015.06–2021.01, School of Chemistry and Chemical Engineering, Nanjing University, Postdoctoral Fellow (Part-time)
•2015.12–Present, School of Pharmacy, Jiangsu University, Associate Professor and Master's Supervisor
Part 5 Research Interests
1.Sample Pretreatment Technologies: Efficient extraction of targets from complex matrices and anti-interference strategies.
2.Novel CRISPR-based Detection Methods: Exploration of Cas12a/Cas13a cis- and trans-cleavage mechanisms and development of isothermal amplification-coupled systems.
3.Novel Ago-based Detection Methods: Construction of Ago activity regulation systems and multiplexed nucleic acid detection strategies.
4.POCT Applications: Development of multiplexed lateral flow assay (LFA) strips, nanoprobe labeling, and instrument-free clinical diagnostic applications.
Part 6 Research Achievements
6.1 Research Projects
• 2018.01–2020.12 | National Natural Science Foundation of China Youth Program "Construction of solid-phase microextraction system based on MOFs electrospun membrane and its application in environmental pollution analysis of antibiotic drugs" (Grant No. 21707053) | NSFC | Principal Investigator
• 2014.07–2017.06 | Jiangsu Provincial Natural Science Foundation Youth Program "Preparation and application of magnetic hollow manganese phosphate-near-infrared fluorescent quantum dot multifunctional nano-targeted drug carriers" (Grant No. BK20140577) | Jiangsu Provincial Natural Science Foundation | Principal Investigator
•2024.11–2029.12 | Longitudinal Project "Development of highly sensitive CRISPR/Ago nucleic acid detection technology for early cancer screening" (Grant No. HX20241144) | Principal Investigator
• 2011.07–2014.06 | Jiangsu Provincial Natural Science Foundation General Program "Applied basic research on multifunctional nanocomposites for rapid detection of foodborne pathogenic microorganisms" | Jiangsu Provincial Natural Science Foundation | Participant (2nd)
• 2011.01–2013.12 | National Natural Science Foundation of China Youth Program "Construction of near-infrared fluorescent QDs-FRET sensor and its application in chemical pesticide research" | NSFC | Participant (2nd)
• 2011.01–2013.12 | National Natural Science Foundation of China Youth Program "Research on molecularly imprinted capillary electrophoresis biomimetic immunoassay based on quantum dot surface" | NSFC | Participant (3rd)
• 2010.01–2012.12 | National Natural Science Foundation of China General Program "TrkA lipid raft chromatography and its application in screening anti-tumor active ingredients of traditional Chinese medicine" | NSFC | Participant (3rd)
• 2009–2010 | Scientific Research Starting Foundation for Returned Overseas Chinese Scholars, Ministry of Education "Study on tetracycline thermosensitive in-situ gel microparticle drug delivery system" | Ministry of Education | Participant (2nd)
• 2024.12–2026.12 | 2024 Drug Regulatory Science Research Program "Rapid and accurate detection of multiple pathogenic bacteria in cosmetics using Ago technology" | Jiangsu Medical Products Administration | Participant (2nd)
• 2013.01–2016.12 | National Natural Science Foundation of China Youth Program "Design, preparation and liver tumor targeting of novel 'triggered' self-assembled protein polymer nanocarriers loaded with bufalin" | NSFC | Participant (5th)
•2014.01–2017.12 | National Natural Science Foundation of China General Program "Differentiation of human iPSC stem cells and their metabolic functions in 3D systems" | NSFC | Participant (6th)
6.2 Publications
1.Wei Cao, Xiang Huang, Fei Ding, Jingwen Wu, Xiaolan Zhu, Li Wang, Multiplexed PfAgo-based digital platform for clinical testing of high-risk HPV subtypes[J]. Analytical Chemistry, 2026, 98 (19) 14473-14481（自然指数期刊，SCI一区，IF=6.7）
2.Sen Tian, Fei Ding, Hui-Yi Wang, Li Wang, Multivalent DNAzyme nanomachine generated by exonuclease III-assisted recycling for fabricating ultrasensitive electrochemical biosensor[J]. Sensors and Actuators B: Chemical, 2025, 399, 134856（SCI一区，IF=8.3）
3.Fei Ding, Xiaomin Hang, Sen Tian, Wei Cao, Jingwen Wu, Li Wang. Nicking endonuclease-mediated primer exchange reaction for rapid and sensitive miRNA detection[J]. Analytica Chimica Acta, 2025, 1351, 343902（SCI二区，IF=6.1）
4.Jiaxin Peng, Honghong Wang, Haixia Cao, Pengfei Liu, Huiyi Wang, Kairen Zhao, Li Wang, A tandem DNA nanomachines-supported electrochemiluminescence assay for attomolar detection of miRNA at ambient-temperature[J]. Chemical Engineering Journal, 2024, 480, 148161. （SCI一区，IF=12.5）
5.Honghong Wang, Pengfei Liu, Jiaxin Peng, Haoming Yu, Li Wang, Poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) modified metal-organic frameworks boosting carbon dots electrochemiluminescence emission for sensitive miRNA detection[J]. Biosensors and Bioelectronics, 2024, 249, 116015（SCI一区，IF=11.8）
6.Jiaxin Peng, Xiaomin Hang, Huiyi Wang, Kairen Zhao, Honghong Wang, Haoming Yu, Li Wang, Low background self-primer EXPAR coupled with colorimetric and lateral flow assay for rapid and sensitive point-of-care detection of miRNA[J]. Sensors and Actuators B: Chemical, 2024, 399, 134856（SCI一区，IF=8.3）
7.Hao-Ming Yu, Guo-Xi Liang, Hui-Yi Wang, Xiao-Min Hang, Hong-Hong Wang, Jia-Xin Peng, Li Wang, A MnO2 nanosheet-mediated CRISPR/Cas12a system for the detection of organophosphorus pesticides in environmental water[J]. Analyst, 2024,149, 729-734.（SCI三区，IF=3.6）
8.Honghong Wang, Pengfei Liu, Jiaxin Peng, Haoming Yu, Li Wang, Label/immobilization-free Cas12a-based electrochemiluminescence biosensor for sensitive DNA detection, Talanta, 2024, 275, 126114（SCI一区，IF=6.7）
9.Xiao-Min Hang, Peng-Fei Liu, Sen Tian, Hui-Yi Wang, Kai-Ren Zhao, Li Wang, Rapid and sensitive detection of Ebola RNA in an unamplified sample based on CRISPR-Cas13a and DNA roller machine[J]. Biosensors and Bioelectronics, 2022, 211, 114393.（SCI一区，IF=11.8）
10.Guo-Xi Liang, Shu-Ying Ye, Hao-Ming Yu, Kai-Ren Zhao, Peng-Fei Liu, Zhi-Jun Liu, Li Wang, A potent fluorescent biosensor integrating 3D DNA walker with localized catalytic hairpin assembly for highly sensitive and enzyme-free ZIKA virus detection[J]. Sensors and Actuators B: Chemical, 2022, 354, 131199. （SCI一区，IF=8.3）
11.Xiao-Min Hang, Kai-Ren Zhao, Hui-Yi Wang, Peng-Fei Liu, Li Wang, Exonuclease III-assisted CRISPR/Cas12a electrochemiluminescence biosensor for sub-femtomolar mercury ions determination[J]. Sensors and Actuators B: Chemical, 2022, 368, 132208. （SCI一区，IF=8.3）
12.Xiao-Min Hang, Kai-Ren Zhao, Peng-Fei Liu, Hui-Yi Wang, Li Wang, Cas12a-Assisted RTF-EXPAR for Accurate, Rapid and Simple Detection of SARS-CoV-2 RNA[J]. Biosensors and Bioelectronics, 2022, 114683.（SCI一区，IF=11.8）
13.Hui-Yi Wang, Peng-Fei Liu, Xiao-Min Hang, Kai-Ren Zhao, Li Wang, CRISPR/Cas12a-based fluorescent biosensor for the detection of cholinesterase activity[J]. Sensors and Actuators B: Chemical, 2022, 368, 132208. （SCI一区，IF=8.3）
14.Peng-Fei Liu, Kai-Ren Zhao, Zhi-Jun Liu, Li Wang, Shu-Ying Ye, Guo-Xi Liang, Cas12a-based electrochemiluminescence biosensor for target amplifcation-free DNA detection[J]. Biosensors and Bioelectronics, 2021, 176, 112954.（SCI一区，IF=11.8）
15.Li Wang, Zhi-Jun Liu, Hai-Xia Cao, Guo-Xi Liang, Ultrasensitive colorimetric miRNA detection based on magnetic 3D DNA walker and unmodified AuNPs[J]. Sensors and Actuators B: Chemical, 2021, 337, 129813. （SCI一区，IF=8.3）
16.Li Wang, Pengfei Liu, Zhijun Liu, Haixia Cao, ShuYing Ye, Kairen Zhao, Guoxi Liang, Jun-Jie Zhu, A dual-potential ratiometric electrochemiluminescence biosensor based on Au@CDs nanoflowers, Au@luminol nanoparticles and an enzyme-free DNA nanomachine for ultrasensitive p53 DNA detection[J]. Sensors and Actuators B: Chemical, 2021, 327, 128890. （SCI一区，IF=8.3）
17.Kai-Ren Zhao, Li Wang, Peng-Fei Liu, Xiao-Min Hang, Hui-Yi Wang, Shu-Ying Ye, Zhi-Jun Liu, Guo-Xi Liang, A signal-switchable electrochemiluminescence biosensor based on the integration of spherical nucleic acid and CRISPR/Cas12a for multiplex detection of HIV/HPV DNAs[J]. Sensors and Actuators B: Chemical, 2021, 346, 130485. （SCI一区，IF=8.3）
18.Li Wang, Kai-Ren Zhao, Zhi-Jun Liu, Yi-Bang Zhang, Peng-Fei Liu, Shu-Ying Ye, Ye-Wang Zhang, Guo-Xi Liang, An “on-off” signal-switchable electrochemiluminescence biosensor for ultrasensitive detection of dual microRNAs based on DNAzyme-powered DNA walker[J]. Sensors and Actuators B: Chemical, 2021, 348, 130660. （SCI一区，IF=8.3）
19.Guo-Xi Liang, Kai-Ren Zhao, Yu-Sheng He, Zhi-Jun Liu, Shu-Ying Ye, Li Wang, Carbon dots and gold nanoparticles doped metal-organic frameworks as high-efficiency ECL emitters for monitoring of cell apoptosis[J]. Microchemical Journal, 2021, 171, 106787（SCI二区，IF=5.2）
20.Zhijun Liu, Li Wang, Pengfei Liu, Kairen Zhao, Shuying Ye, Guoxi Liang, Rapid, ultrasensitive and non-enzyme electrochemiluminescence detection of hydrogen peroxide in food based on the ssDNA/g-C3N4 nanosheets hybrid[J]. Food Chemistry，2021, 357, 129753,（SCI一区，IF=10.4）
21.Li Wang, Pengfei Liu, Zhijun Liu, Kairen Zhao, Shuying Ye, Guoxi Liang, Jun-Jie Zhu, Simple tripedal DNA walker prepared by target-triggered catalytic hairpin assembly for ultrasensitive electrochemiluminescence detection of microRNA,[J]. ACS Sensors, 2020, 5(11): 3584-3590（SCI一区，IF=10.9）
22. Hai-Xia Cao, Peng-Fei Liu, Li Wang, Zhi-Jun Liu, Shu-Ying Ye, Guo-Xi Liang, Nonenzymatic chemiluminescence detection of circulating tumor cells in blood based on Au@luminol nanoparticles, hybridization chain reaction and magnetic isolation[J]. Sensors and Actuators B: Chemical, 2020, 318, 128287.（SCI一区，IF=8.3）
23.Pengfei Liu, Li Wang, Kairen Zhao, Zhijun Liu, Haixia Cao, Shuying Ye, Guoxi Liang, High luminous efficiency Au@CDs for sensitive and label-free electrochemiluminescent detection of circulating tumor cells in serum[J]. Sensors and Actuators B: Chemical, 2020, 316, 128131.（SCI一区，IF=8.3）
